Poster abstracts
Poster number 116 submitted by Sun Jeong Im
SPR-SELEX: New Method to Determine DNA Aptamers that Targets E.coli for Biosensor
Sun Jeong Im (Department of Chemistry, Southern Illinois University Edwardsville), Hailey Bowen (Department of Chemistry, Southern Illinois University Edwardsville), Mina Sumita (Department of Chemistry, Southern Illinois University Edwardsville)
Abstract:
Escherichia coli (E. coli) outbreak is a serious health issue in the United States today. These outbreaks are caused by pathogenic E. coli strains. One possible way to prevent outbreaks is by detecting the harmful E. coli via biosensor with a DNA aptamer. Using the systematic evolution of ligands by exponential enrichment (SELEX) technique, the DNA aptamer sequence with a high affinity and specificity to the target E. coli strain K12 can be determined. However, the current SELEX procedure is time-consuming. Also, the selection progress cannot be monitored in real time with the conventional SELEX method without radiolabeling. Surface plasmon resonance (SPR) can resolve these disadvantages of the current SELEX technique by measuring the molecular binding kinetics in real-time without any labeling. In this project, we present the development of a faster and safer SELEX method combined with surface plasmon resonance (SPR-SELEX) to identify DNA aptamers.
Keywords: SELEX, SPR