Poster abstracts
Poster number 153 submitted by Christina Psihountas
Using LC-MS/MS to detect tRNA modification profile differences in Bacillus subtilis
Christina Psihountas (UC Chemistry), Manasses Jora (UC Chemistry), Patrick A. Limbach (UC Chemistry)
Abstract:
Transfer Ribonucleic Acids (tRNAs) have more modified bases than the other species of RNA. Temperature, culture media, stress, and cell development are suspected to influence tRNA nucleoside modifications in a qualitative and/or quantitative nature. Current databases do not reflect modification changes or detail the culturing conditions of obtained data. Changes in tRNA modifications are being studied in the gram positive and spore forming bacteria, Bacillus subtilis. Here we show a comparison of the tRNA profiles of vegetative cells and spores. A comparison of profiles between domesticated and undomesticated strains are also discussed. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), we can detect and characterize modifications from total tRNA samples obtained at different phases of the cell life cycle. Abundance of nucleosides from Bacillus subtilis is being analyzed to learn the effects of culturing conditions on the tRNA profile.
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Keywords: tRNA, Nucleosides, Mass Spectrometry