Poster abstracts

Poster number 29 submitted by Catherine Musselman

Impact of Static and Dynamic A-form Heterogeneity on the Determination of RNA Global

Catherine Musselman (University of Michigan), Kush Gulati (University of Michigan), Lesley Foster (University of Michigan), Hashim M. Al-Hashimi (University of Michigan)

Abstract:
We examined how static and dynamic deviations from the idealized A-form helix propagate into errors in the principal order tensor parameters determined using residual dipolar couplings (rdcs). A 20 ns molecular dynamics (MD) simulation of the HIV-1 transactivation response element (TAR) RNA together with a survey of spin relaxation studies of RNA dynamics reveals that pico-to-nanosecond local motions in non-terminal Watson-Crick base-pairs will uniformly attenuate base and sugar one bond rdcs by ~7%. Gaussian distributions were generated for base and sugar torsion angles through statistical comparison of 40 RNA X-ray structures solved to < 3.0 Å resolution. For a typical number of rdcs (≥ 11), these structural deviations together with rdc uncertainty (1.5 Hz) lead to average errors in the magnitude and orientation of the principal axis of order that are < 9% and < 4º respectively. The errors decrease to < 5% and < 4º for ≥ 17 rdcs. A protocol that allows for estimation of error in A-form order tensors due to both angular deviations and rdc uncertainty (Aform-RDC) is validated using theoretical simulations and used to analyze rdcs measured previously in TAR in the free state and bound to four distinct ligands. Results confirm earlier findings that the two TAR helices undergo large changes in both their mean relative orientation and dynamics upon binding to different targets.

Keywords: Alignment Tensor Error, Idealized A-form Helix, RDC