Poster abstracts

Poster number 65 submitted by Peng Yao

Alternative polyadenylation in EPRS coding region generates a truncated protein that maintains a “translational trickle” from GAIT target mRNAs

Peng Yao (Cell Biology Department, Lerner Research Institute, Cleveland Clinic), Paul Fox (Cell Biology Department, Lerner Research Institute, Cleveland Clinic)

Abstract:
The GAIT (Gamma-interferon Activated Inhibitor of Translation) complex inhibits translation of inflammatory transcripts in human myeloid cells, thereby preventing excessive accumulation of potentially harmful proteins. During the inhibition phase we observed a low-level “trickle” of translation of GAIT target mRNAs independent of mRNA amount. In circumstances where inflammatory proteins also have protective roles, e.g., vascular endothelial growth factor-A (VEGF-A), complete knock-down can cause tissue injury and tumor growth. Thus, incomplete inhibition of gene expression by the GAIT system might be beneficial. We discovered a novel mechanism that compels low-level translation of GAIT target mRNAs despite robust inhibition by the GAIT system. Glutamyl-prolyl tRNA synthetase (EPRS) is the sole component of the GAIT complex that binds the GAIT element-bearing mRNAs. We found a truncated form of EPRS, termed \

Keywords: alternative polyadenylation, translational trickle, VEGF-A