Poster abstracts

Poster number 46 submitted by Man Liu

Lateral Root Development Associated with a Polyadenylation Factor in Arabidopsis

Man Liu (Department of Botany, Miami University), Xiaohui Wu (Department of Botany, Miami University), Arthur G. Hunt (Department of Plant and Soil Sciences, University of Kentucky), Qingshun Quinn Li (Department of Botany, Miami University)

Abstract:
Alternative polyadenylation plays an important role in gene expression regulation in eukaryotes. We previously reported that an Arabidopsis cleavage and polyadenylation specificity factor 30 (AtCPSF30) is involved in posttranscriptional processing to control the responses of plants to oxidative stresses, and the mutation of AtCPSF30, called oxt6, can affect the poly(A) site selection (Zhang et al., PLoS One, 3:e2410). We further found that this oxt6 mutant has drastically reduced lateral roots. Upon examination of the lateral root primordium development, the results showed that the reduction is mainly on stage I to III in oxt6. How AtCPSF30 regulates root development, however, is not known. Here, we have developed a deep sequencing method for the identification of polyadenylated RNA termini with poly(A) tags (PAT-seq), and applied this method to investigate alternative polyadenylation in the root development. PAT-seq identified 283 genes showed different alternative polyadenylation patterns in WT and oxt6. Among them, 25 are known genes that are involved in lateral root development. These findings provide candidate genes for studying post-transcriptional regulation in lateral root development.

Keywords: alternative polyadenylation, AtCPSF30, root development