Poster abstracts

Poster number 1 submitted by Amrita Bandyopadhyay

Insights into the plant CstF complex

Amrita Bandyopadhyay (Plant and Soil Sciences, University of Kentucky), Arthur G. Hunt (Plant and Soil Sciences, University of Kentucky)

Abstract:
3’ end modification by polyadenylation is a ubiquitous feature of almost all of the eukaryotic mRNA species. This process involves the functioning of a complex unit of polyadenylation factors, the enzymes which catalyze the whole process. Cleavage Stimulation Factor (CstF) is a heterotrimer complex of the polyadenylation unit composed of CstF50, CstF64 and CstF77. This study highlights some aspects of the Arabidopsis thaliana homolog of CstF64, playing a vital role in RNA recognition and on the other hand in transcription termination and polyadenylation.Yeast two-hybrid interaction studies showed a novel kind of interaction of CstF64 with FIP1. It is also speculated from sub-cellular localization by agroinfiltration that CstF64 is co-localized to the nucleus in presence of CstF77, which is known to be the only CstF subunit having the nuclear localization signal in mammals. Based on these studies, a model for a sub-complex involving CstF64, CstF77 and FIP1 may be built. The ramifications of this model will be presented and discussed.

References:
Li Q, Hunt AG(1997)The Polyadenylation of RNA in Plants.Plant Physiol.115:321-325

Takagaki Y, Manley JL(2000)Complex Protein Interactions within the Human Polyadenylation Machinery Identify a Novel Component. Mol.Cell.Biol.20:1515-1525

Keywords: Polyadenylation, Cleavage Stimulation Factor (CstF), Yeast two-hybrid and Agroinfiltration