RRM
2008 Rustbelt RNA Meeting
RRM
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Poster abstracts
Abstract:
In eukaryotes, 3?f end processing is an important post-transcriptional modification that results in mature mRNA. In plants, it is guided by three consensus elements, the far upstream element (FUE), near upstream element (NUE) and cleavage / polyadenylation site. This process is mediated by interactions between the protein and RNA as well as by protein-protein interactions. The key complexes involved in the poly (A) tail addition are Cleavage and Polyadenylation Specificity Factor (CPSF) and Cleavage and Stimulation Factor (CstF). These complexes are made up of multiple subunits. In mammals the cleavage stimulation factor is composed of three subunits, CstF50, CstF64 and CstF77. The present study focuses on the orthologues of mammalian CstF50 in Arabidopsis. CstF50 is a WD-repeat containing protein; Arabidopsis has two possible orthologues of CstF50. One of these orthologues (FY) has been shown to be involved in flowering while the other orthologue (At5g60940) has not been studied. In this regard, we show that AtCstF50 is essential and plays a different role from its mammalian counterpart. Further studies focus on its role in seed development.
In Arabidopsis FY is recruited to the mRNA by FCA, an RNA binding protein. FY in turn recruits 3?fend processing complex and mediates the polyadenylation reaction. We are testing this model using a tethering assay. Preliminary data in these lines will be presented.
References:
1. Li, Q. and A. G. Hunt (1997). The Polyadenylation of RNA in Plants. 115: 321-325.
2. Y Takagaki, J L Manley, C C MacDonald, J Wilusz, and T Shenk (1990) A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs.
3. Richard M. Amasino (2003). Flowering time: a pathway that begins at the 3?Πend. vol 13 Issue 17.
Keywords: 3fend processing,CstF, FY, FCA, and tethering assay
