Talk abstracts

Talk on Friday 03:30-03:45pm submitted by Jey Sabith Erbon

Regulation of Androgen Receptor expression through its 3’ untranslated region

Jey Sabith Ebron (Center for Gene regulation in Health and Disease, Cleveland State University), Kavleen Sikand, Girish C. Shukla

Abstract:
Androgen receptor (AR) is a transcription factor and a member of the steroid receptor family. AR plays an important role in the regulation of both prostate cell proliferation and growth suppression. AR is a major target for Prostate Cancer treatment. AR mRNA contains a 6.8 kb long 3’ untranslated regions (UTR) and it is a target of many miRNAs. Targeting of AR by miRNAs is likely to modulate differential regulation of AR expression. We have shown that AR is a target of miR-488* and miR-644a. In this study, we are investigating the combinatorial action of miRNAs on AR expression. The repression of AR expression is substantially enhanced in prostate cancer cells treated with both miRNAs in comparison with the individual miRNAs. Similar additive effect was also observed in cancer cell apoptosis and viability. In addition, we are investigating effects of AR 3’ UTR in AR expression using luciferase reporter system. Preliminary data demonstrates that the expression of Luciferase containing longer UTRs is suppressed. In summation, our data indicates the significance of miRNA and UTR in AR gene regulation at posttranscriptional levels.

Keywords: UTR, miRNA, Androgen Receptor