Poster abstracts

Poster number 78 submitted by Yizhu Lin

Human lncRNA Structure Probing by High-throughput Sequncing Method

Yizhu Lin (Department of Biological Sciences, Carnegie Mellon University), C. Joel McManus (Department of Biological Sciences, Carnegie Mellon University)

Abstract:
In recent years, long noncoding RNAs (lncRNAs) have been increasingly recognized as important regulators of gene expression in various levels. The human genome encodes thousands of lncRNAs, and an increasing number of these have been associated with human diseases. lncRNA structures and lncRNA-protein interactions are expected to play essential roles in their gene gene regulatory functions, however, our knowledge about that is limited. To address this issue, we have developed a novel method for high-throughput probing of RNA structure using massively parallel sequencing (Mod-seq). Compared to traditional RNA structure probing methods, Mod-seq provides substantial increases in throughput, allowing rapid and simultaneous probing of the whole transcriptome. In addition, Mod-seq results in quantitative analysis of lncRNA structure. By comparing Mod-seq results in vivo and in vitro, we aim to identify putative lncRNA-protein interactions.

Keywords: RNA Structure, lncRNA