Poster abstracts
Poster number 104 submitted by Christina Psihountas
tRNA modification profile changes during growth phase transitions in Bacillus subtilis
Christina Psihountas (Chemistry Department, University of Cincinnati), Manasses Jora (Chemistry Department, University of Cincinnati), Patrick A. Limbach (Chemistry Department, University of Cincinnati)
Abstract:
Transfer Ribonucleic Acids (tRNAs) have more modified bases than the other species of RNA. Temperature, culture media, stress, and cell development are suspected to influence tRNA nucleoside modifications in a qualitative and/or quantitative nature. Current databases do not reflect modification changes or detail the culturing conditions of obtained data. Changes in tRNA modifications are being studied in the gram positive and spore forming bacteria, Bacillus subtilis. Here we show that there is a change in the tRNA profile as the bacteria progresses through its life cycle. This method will also be used to monitor tRNA profiles during sporulation. We hypothesize that we will see a change in nucleoside abundance when vegetative cell and spore tRNA profiles are compared. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), we can detect and characterize modifications from total tRNA samples obtained at different phases of growth and during sporulation.
References:
El Yacoubi, Basma, Marc Bailly, and Valérie de Crécy-Lagard. 2012. Annual Review of Genetics 46(1): 69-95.
Xiao Wang and Chuan He. 2014. Molecular Cell 56: 5-12.
K. Okamoto and B.S. Vold. 1992. Biochimie 74: 613-618.
Yun-Hua Jeng and Roy H. Doi. 1975. Journal of Bacteriology, 121(3): 950-958
Meyer, F., et al. 2008. BMC Bioinformatics 9(1): 386.
Keywords: growth phase, tRNA modifications, LCMS