Poster abstracts
Poster number 50 submitted by Sun Jeong Im
Using Surface Plasmon Resonance (SPR) to Determine DNA Aptamers that Binds to E. coli for Biosensor Development
Sun Jeong Im (Department of Chemistry, SIUE), Hailey Bowen (Department of Chemistry, SIUE), Mina Sumita (Department of Chemistry, SIUE)
Abstract:
Pathogenic Escherichia coli (E. coli) strains can cause food poisoning. One possible way to prevent food poisoning is by detecting the harmful E. coli via biosensor with DNA aptamer. The DNA aptamer is selected using a method called systematic evolution of ligands by exponential enrichment (SELEX). However, the SELEX method is time consuming when identifying the aptamer. Therefore, we are developing SELEX with surface plasmon resonance (SPR-SELEX). SPR technique can select and detect the affinity to the target molecule in the same time to simplify the SELEX process. The aptamer selection against E. coli with SPR-SELEX will proceed for the future biosensor.
Keywords: Aptamer, SELEX, SPR