Poster abstracts
Poster number 142 submitted by Hannah Talbot
Encoding, Decoding, and Rendering Information in DNA Nanoswitch Libraries
Hannah Talbot (RNA Institute, University at Albany), Ken Halvorsen, Arun Richard Chandrasekaran (RNA Institute, University at Albany)
Abstract:
DNA-based construction allows the creation of molecular devices that are useful in information storage and processing. Here, we combine the programmability of DNA nanoswitches and stimuli-responsive conformational changes to demonstrate information encoding and graphical readout using gel electrophoresis. We encoded information as 5-bit binary codes for alphanumeric characters using a combination of DNA and RNA inputs that can be decoded using molecular stimuli such as a ribonuclease. We also show that a similar strategy can be used for graphical visual readout of alphabets on an agarose gel, information that is encoded by nucleic acids and decoded by a ribonuclease. Our method of information encoding and processing could be combined with DNA actuation for molecular computation and diagnostics that require a nonarbitrary visual readout.
References:
H Talbot, K Halvorsen & AR Chandrasekaran
ACS Synthetic Biology, 12: 978 (2023)
MA Koussa et al. Nature Methods 12: 123 (2015).
AR Chandrasekaran, J Zavala and K Halvorsen.
ACS Sensors 1 120 (2016).
Keywords: DNA nanoswitch, DNA nanostrutures, Data storage