Poster abstracts
Poster number 81 submitted by Sineth G Kodikara
Detecting secondary structure formation with FRET-PAINT
Sineth G. Kodikara (Department of Physics, Kent State University), Kylie Merkel (Department of Physics, Kent State University), Simon Haas (Department of Physics, Kent State University), Sajad Shiekh (Department of Physics, Kent State University), Hamza Balci (Department of Physics, Kent State University)
Abstract:
We present single-molecule studies demonstrating the capabilities of the FRET-PAINT method to detect secondary structures that would be challenging to detect with alternative methods, particularly single-molecule FRET (smFRET). Instead of relying on the change in end-to-end separation as in smFRET, we use the change in accessibility to a small probe as the criterion for secondary structure formation and relative stability. As a model system, we study G-triplex formation by human telomeric repeat sequences in different structural contexts.
Keywords: G-Triplex, FRET-PAINT