Poster abstracts
Poster number 59 submitted by Mohammed Hassan
Quantification of RNA nanoparticles in the eye tissues using LC-MS
Mohammed Hassan (Chemistry Department / University of Cincinnati), Kevin Li (College of Pharmacy / University of Cincinnati), Patrick Limbach (Chemistry Department / University of Cincinnati)
Abstract:
Effective ocular drug delivery for treatment chronic posterior eye diseases remains a challenging task. RNA nanoparticles, with defined size and shape, could be used as vectors for enhancement of delivery for RNA-based therapeutics. Moreover, they can be fabricated to allow simultaneous targeting and/or delivery of multiple therapeutic and targeting ligands. Consequently, this could be used to achieve synergistic effects for drug delivery and therapy. On the other hand, quantification of small RNA molecules in a complex sample is challenging as well. However, PCR is the gold standard for RNA quantification, it doesn’t work with small RNA. Here, This first report for LC-MS assay for quantification of RNA nanoparticles in the murine eye tissues after subconjunctival injection in the mouse eye. Our results show that the limit of detection is in femtomolar range. This assay would open the door for starting the pre-clinical studies on the delivery of RNA nanoparticles to the eye.
References:
1. Urtti A. Challenges and obstacles of ocular pharmacokinetics and drug delivery. Adv Drug Deliv Rev. 2006;58:1131–1135.
2. Lee SS, Robinson MR. Novel drug delivery systems for retinal diseases. A review. Ophthalmic Res. 2009;41:124–135
3. Khisamutdinov EF, Li H, Jasinski DL, Chen J, Fu J, Guo P. Enhancing immunomodulation on innate immunity by shape transition among RNA triangle, square and pentagon nanovehicles. Nucleic Acids Res. 2014;42:9996–10004.
4. Jasinski DL, Khisamutdinov EF, Lyubchenko YL, Guo P. Physicochemically tunable polyfunctionalized RNA square architecture with fluorogenic and ribozymatic properties. ACS Nano. 2014;8:7620–7629.
Keywords: LC-MS, Quantification , RNA