Poster abstracts
Poster number 63 submitted by Hannah Hertz
Investigating the role of kinetochore component HCP-1 perinuclear condensates for successful cell division in C. elegans
Hannah L. Hertz (Biological Chemistry and Pharmacology, The Ohio State University), Ian F. Price (Biological Chemistry and Pharmacology, The Ohio State University), Wen Tang (Biological Chemistry and Pharmacology, The Ohio State University)
Abstract:
In C. elegans, HCP-1 and HCP-2 are key components of the kinetochore which initiates central spindle formation and promotes proper chromosome segregation during mitosis. Similar to their mammalian counterpart, HCP-1/2 exhibit dynamic localization patterns, transitioning from the nuclear envelope during prophase to kinetochores during cell division. Although the localization of HCP-1 at kinetochores is well studied, less is known why HCP-1 localizes to the nuclear envelope. Here we seek to characterize this dual localization of HCP-1 throughout the cell cycle. We found prior to cell division HCP-1 is stored in condensate-like foci at the nuclear envelope along with nucleoporins. Notably, perinuclear localization of HCP-1 is dependent on the nucleoporin NPP-14. By disrupting perinuclear HCP-1 localization in npp-14 mutants, our project seeks to elucidate whether perinuclear HCP-1 is essential for its subsequent recruitment to kinetochores and the proper assembly of the central spindle. Moreover, we seek to address whether proper perinuclear localization of HCP-1 in adults is required for proper partitioning of HCP-1 and successful mitosis in their progeny.
Keywords: Condensates, Kinetochore