Talk abstracts
Talk on Friday 05:00-06:00pm submitted by Phil Bevilacqua
Keynote lecture: Understanding RNA folding in vivo by two complementary approaches
Philip C. Bevilacqua (Departments of Chemistry and of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Penn State University), McCauley Meyer (Department of Biochemistry and Molecular Biology, Center for RNA Molecular Biology, Penn State University), Saehyun Choi, Christine D. Keating (Department of Chemistry, Penn State University), Jacob P. Sieg (Department of Chemistry, Center for RNA Molecular Biology, Penn State University), Sarah M. Assmann (Department of Biology, Center for RNA Molecular Biology, Penn State University), Ryota Yamagami (Department of Chemistry, Center for RNA Molecular Biology, Penn State University)
Abstract:
RNA has an exceptionally diverse and important array of functions, which are driven by RNA folding in the cell. There is thus great interest in understanding how RNA folds in the cell. One approach is chemical probing of RNA in vivo and transcriptome-wide. Another approach is preparing in vivo-like conditions and measuring RNA folding. I will describe the advantages and limitations of each approach and lay out some of the studies from our lab on chemical probing of RNA in vivo over the last decade.
References:
1. Meyer, M. O., Choi, S., Keating, C. D., Bevilacqua, P. C. & Yamagami, R. (2023). Structure-seq of tRNAs and other short RNAs in droplets and in vivo. Methods Enzymol 691, 81-126.
2. Yamagami, R., Sieg, J. P., Assmann, S. M. & Bevilacqua, P. C. (2022). Genome-wide analysis of the in vivo tRNA structurome reveals RNA structural and modification dynamics under heat stress. Proc Natl Acad Sci U S A 119, e2201237119.
3. Meyer, M. O., Yamagami, R., Choi, S., Keating, C. D. & Bevilacqua, P. C. (2023). RNA folding studies inside peptide-rich droplets reveal roles of modified nucleosides at the origin of life. Sci Adv 9, eadh5152.
Keywords: tRNA, structure-seq, in vivo