Poster abstracts
Poster number 96 submitted by Yaseswini Neelamraju
The human RBPome: from genes and proteins to human disease
Yaseswini Neelamraju (Department of Biohealth Informatics,Indiana University-Purdue University,Indianapolis), Seyedsasan Hashemikhabir (Department of Biohealth Informatics,Indiana University-Purdue University,Indianapolis), Sarath Chandra Janga (Department of Biohealth Informatics,Indiana University-Purdue University,Indianapolis)
Abstract:
RNA Binding Proteins (RBPs) play a central role in mediating post transcriptional regulation of genes. However less is understood about them and their regulatory mechanisms. In this study, we construct a repertoire of 1344 RBPs identified from several experimental studies and present a comprehensive meta-analysis to understand their characteristics at a global scale. The domain architecture of RBPs enabled us to classify them into three groups - Classical (29%), Non-classical (19%) and Unclassified (52%). A higher percentage of proteins with unclassified domains reveal the presence of various uncharacterised motifs that can potentially bind RNA. RBPs were found to be highly disordered compared to non-RBPs (p<2.2e-16, Fisher’s exact test), indicating a dynamic regulatory role of RBPs in cellular functioning. Evolutionary analysis in 62 different species showed that RBPs are highly conserved compared to non-RBPs (p<2.2e-16, Wilcox-test), reflecting the conservation of various post-transcriptional processes like mRNA splicing and translational control. Expression patterns of RBPs from human proteome map revealed that majority (~60%) of the RBPs are tissue-specific. RBPs were also seen to be highly associated with several neurological disorders, cancers and inflammatory diseases. Further, anatomical context analysis revealed that B cells, T-cells, Fetal Liver and Fetal Brain are enriched in diseases associated with RBPs implying a prominent role for RBPs in immune responses and different developmental stages. These analyses are made accessible to researchers in the form of a database called RBP expression and disease dynamics database (READ DB: http://darwin.soic.iupui.edu/)
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Keywords: RNA Binding proteins, post transcriptional regulation