Poster abstracts
Poster number 117 submitted by Lauren Schultz
Effects of ionizing radiation on the RNA of fungi
Lauren Schultz (Department of Chemistry, University of Cincinnati)
Abstract:
Exposure to ionizing radiation in radiotrophic fungi has been shown to cause changes in the regulation of various genes, including some known to be involved in RNA modification. Here we are investigating how ionizing radiation may impact RNA modification levels using the radiotrophic fungi Cladosporium shaperospermum. Initial experiments involve identifying total RNA modification profiles of C. sphaerospermum by LC-MS/MS. C. sphaerospermum samples, both irradiated at varying dosages with 60Co and non-irradiated, are analyzed using the same procedure. Our preliminary findings reveal a change in RNA modification profiles for samples exposed to ionizing radiation as compared to unexposed controls. These differences require further studies to determine any role they may play in the organism’s radiotrophism.
References:
[1] Robertson KL, Mostaghim A, Cuomo CA, Soto CM, Lebedev N, et al. Adaption of the Black Yeast Wangiella dermatitidis to Ionizing Radiation: Molecular and Cellular Mechanisms. PloS ONE, 2012. 7(11): e48674.
Keywords: Radiation, Fungi, Mass spectrometry