Poster abstracts
Poster number 10 submitted by Karan Bedi
Genome-wide analysis of splicing kinetics reveals differential rates of splicing and turnover for mammalian introns
Karan Bedi (Department of Radiation Oncology, University of Michigan), Jayendra Prasad (Department of Radiation Oncology, University of Michigan), Brian Magnuson (Department of Radiation Oncology, University of Michigan), Artur Veloso (Department of Radiation Oncology, University of Michigan), Michelle T Paulsen (Department of Radiation Oncology, University of Michigan), Mats Ljungman (Department of Radiation Oncology, University of Michigan)
Abstract:
Splicing is carried out by spliceosomes and involves splice sites recognition, removal of introns and ligation of exons. Components of the spliceosome interact with the elongating RNA polymerase II (RNAPII) allowing for splicing concurrent with transcription. Here we used BruChase-seq to obtain temporal resolution of splicing kinetics genome-wide and found that the emergence of completely spliced transcripts in the nascent RNA pool was surprisingly slow and variable even within transcripts. Minor class introns were spliced out slower than major class introns and some transcripts, such as from ribosomal protein-coding genes, were rapidly spliced in a spliceosome-independent manner. Slowly spliced introns had an increased density of RNA polymerase II suggesting that transcription elongation through these introns occurred at a slower rate than for other introns. Together these genome-wide studies suggest that splicing and post-splicing turnover occur at different rates for different introns revealing a novel layer of gene regulation.
Keywords: BruChase-Seq, splicing, intron turnover