Workshop 1: Coping Strategies as Professional Development
We will discuss the effect of stress on our ability to do science and explore the use of Social-Emotional Learning (SEL)-based coping strategies to improve focus, learning and execution, to optimize time management, and to engage others effectively in stressful situations. Attendees will be introduced to these skills, participate in experiential skills practice, and develop a plan to integrate skills into their professional development. The ultimate goal is to help trainees to mitigate stress, thereby improving their experience of training in science.
1. Describe the rationale for incorporating Social-Emotional Learning into your development as scientists.
2. Identify at least three coping strategies that can help to manage stress.
3. Develop a plan to incorporate coping strategies into professional (and personal) life.
4. Describe and apply components of the interpersonal effectiveness skill “DEAR MAN”.
Organizers: Donny D. Licatalosi, Ph.D., Associate Professor, and Frank Tedeschi, Ph.D., Research Scientist, Case Western Reserve University, Center for RNA Science and Therapeutics
RNA binding proteins (RBPs) have critical roles in all cells. Determining the functions of a given RBP requires identification of it’s in vivo RNA targets. Cross-linking and immunoprecipitation (CLIP) combined with deep sequencing provides an opportunity to create high resolution transcriptome-wide maps that of RBP-RNA contact sites in cells.
This workshop will present an overview of the CLIP methodology from purification of protein-RNA complexes to analysis of sequencing data. In the first part of the workshop, Dr. Licatalosi will describe key steps in the CLIP protocol including experimental considerations, optimization, troubleshooting, and the types of information that CLIP can (and can’t) provide. In the second part of the workshop, Dr. Tedeschi will describe computational approaches to analyze CLIP sequencing data including different processing workflows, file formats, and visualization techniques.