Poster abstracts

Poster number 152 submitted by Nicole Burbach

Purification with

Nicole Burbach (Department of Chemistry, Southern Illinois University Edwardsville), Anthony DeGregorio (Department of Chemistry, Southern Illinois University Edwardsville), Mina Sumita (Department of Chemistry, Southern Illinois University Edwardsville)

Purification is an important step of organic synthesis. Column chromatography is a typical method of purifying products; however, it is difficult when there are several side products and if the Rf values are similar to one another. The early step of 2-aminopyridine nucleoside synthesis faces this challenge. At the base protection step, the unused 1,2-bis(chlorodimethylsilyl)ethane was difficult to separate from the target product. Because of the high boiling point of the protecting group, we decided to use Kugelrohr distillation. We successfully house-built a cost-effective Kugelrohr distillation apparatus using parts of an old rotovap. We achieved the desired purified product and are now ready for the next step of the synthetic scheme.

Keywords: RNA modification, Nucleoside Synthesis, Kugelrohr distillation