Poster abstracts

Poster number 153 submitted by Anthony Degregorio

Synthesis of 1-Aminopyridine for Fluorescent Studies of RNA

Anthony DeGregorio (Department of Chemistry, Southern Illinois University Edwardsville), Nicole Burbach (Department of Chemistry, Southern Illinois University Edwardsville), Ashlynn Winkler (Department of Chemistry, Southern Illinois University Edwardsville), Mina Sumita (Department of Chemistry, Southern Illinois University Edwardsville)

RNA has multiple different functions and compositions, which allow it to have a major role in many important biological processes. Fluorescence spectroscopy has advantages including its sensitivity. However, regular nucleosides are not fluorescence active at room temperature. Therefore, it is required to synthesize fluorescent modified nucleotides in studying RNA function and composition. While there is an available fluorescent modified purine nucleoside analog, there is not a fluorescent nucleoside analog for pyrimidine nucleosides. In this research project, a fluorescent pyrimidine analog, 2-aminopyridine nucleoside, is developed for further fluorescence studies of RNA.

Jones, A.C.; Neely, R.K., 2-Aminopurine as a fluorescent probe of DNA conformation and the DNA-enzyme interface. Quarterly reviews of Biophysics 2015, 48(2), 244-79.

Hanessian, S., and Machaalani, R. A Highly Stereocontrolled and Efficient Synthesis of Alpha- and Beta-Pseudouridine, 2003, 44, 2049-2052.

Keywords: RNA modification, Fluorescence Spectroscopy, Nucleoside Synthesis