Poster abstracts
Poster number 57 submitted by Phillip McCown
Improved long non-coding RNA annotation and detection in human transcriptomic profiles
Phillip J. McCown (Department of Internal Medicine, University of Michigan), Felix Eichinger (Department of Internal Medicine, University of Michigan), Matthew Manninen (Department of Internal Medicine, University of Michigan), Fadhl Alakwaa (Department of Internal Medicine, University of Michigan), Damian Fermin, Abhijit S. Naik, Sean Eddy (Department of Internal Medicine, University of Michigan), Matthias Kretzler (Department of Internal Medicine, University of Michigan)
Abstract:
The importance of long non-coding (lnc) RNA involvement in biology is increasingly recognized. By remapping transcriptomic datasets from human kidneys, we can identify lncRNAs involved in disease processes with cell type selectivity. To accomplish this, we created the lEG (lncRNAKB-Ensembl GTF), a non-redundant, modified Gene Transfer File (GTF) that incorporates lncRNAs from the lncRNAKB and Ensembl. The lEG was then applied to bulk RNA-seq and single cell RNA-seq (scRNA-seq) analyses and processing pipelines.
Keywords: lncRNA, Single cell RNA-seq, Kidney