Poster abstracts
Poster number 63 submitted by James Londino
Examining the Role of Transfer RNA in the Response to Infection
James Londino (The Ohio State University, Internal Medicine), Kelsey Kershner (The Ohio State University, Internal Medicine), Bryan Miller (The Ohio State University, Internal Medicine), Mithu De (The Ohio State University, Internal Medicine)
Abstract:
The role of transfer RNA in the response to infection has not explored. To determine how tRNA modifies the response to infection, we created a tRNA expression library encompassing 150 tRNA isodecoders under their endogenous promoters. In addition, we created vectors expressing 22 mitochondrial tRNA with leader sequences previously demonstrated to ensure mitochondrial localization and processing. Using nanopore sequencing, we confirmed that individual isodecoders were increased following transfection. In a high-throughput assay, we examined the effect of individual tRNA on cell viability and cytokine expression in response to various inflammatory stimuli including TNFa, influenza PR8, and pseudomonas aeruginosa. We determined that overexpression of several tRNA significantly altered the production of the inflammatory cytokine IL-6 in response to several stimuli. In future studies, we will examine the role of candidate tRNA on the inflammatory response in primary airway epithelium and in intact lung tissue sections.
Keywords: tRNA, cytokines, infection