Poster abstracts
Poster number 39 submitted by Jey Sabith Ebron
Regulation of Androgen Receptor expression through its 3’ UTR
Jey Sabith Ebron (Center for Gene regulation in Health and Disease, Department of Biological, Geo and Evs Sciences, Cleveland State University), Kavleen Sikand , Girish C. Shukla (Center for Gene regulation in Health and Disease, Department of Biological, Geo and Evs Sciences, Cleveland State University)
Abstract:
Androgen receptor (AR) is a member of the steroid receptor family which plays a significant role in the regulation of both prostate cell proliferation and growth suppression. AR is a target for Prostate Cancer (PCa) treatment. The 3’UTR of AR mRNA is 6.8 kb long and is a potential target of many miRNAs. We have identified a novel regulation of AR signaling pathway and invasion through regulation of AR gene expression using miRNAs. In this study we show that AR is a direct target of hsa-miR-644 and hsa-miR-644. Our results demonstrate that hsa-miR-644 targets AR signaling pathway as well as migration and invasion of PCa cells. Further, we show the synergistic and combinatorial action of hsa-miR-488* and hsa-miR-644 on AR gene expression in prostate cancer cells. In addition, we have explored the inhibitory function of RNA binding protein HuR on the miRNA mediated repression of AR expression in PCa cells. Our preliminary results indicate that HuR affects the downregulation of AR presumably by competing for miRNA binding target sites within the 3’ UTR. These results demonstrate a complex posttranscriptional regulation of AR that is mediated via miRNAs and HuR protein antagonistic action in PCa cells.
Keywords: Androgen receptor, miRNA, Prostate Cancer