Poster abstracts
Poster number 63 submitted by Daniel Jasinski
RNA as a boiling-resistant anionic polymer material to build robust polygons with tunable angle, size, stoichiometry and functionality
Daniel L. Jasinski (Nanobiotechnology Center, Markey Cancer Center, and College of Pharmacy, University of Kentucky), Emil F. Khisamutdinov (Department of Chemistry, Ball State University), Peixuan Guo (Nanobiotechnology Center, Markey Cancer Center, and College of Pharmacy, University of Kentucky)
Abstract:
RNA, a special class of anionic polymer, has recently emerged as an important nanotechnology platform due to its diversity, versatility and thermostability. RNA nanoparticles can be fabricated with precision characteristic of DNA while possessing adaptable tertiary structures and catalytic functions that can mimic some forms of proteins. RNA is unique in comparison to DNA by virtue of its high thermodynamic stability, ability to form both canonical and noncanonical base pairings, capability of base stacking, and unique in vivo attributes in therapeutic applications. RNA nanotechnology involves bottom-up approaches to assemble nanometer-scaled particles composed of primarily RNA. Utilizing the three-way junction (3WJ) motif from the phi29 motor pRNA we have constructed an assortment of thermodynamically and chemically stable RNA nanoparticles that carry multiple therapeutics, fluorogenic motifs, and other functional modules. The nanoparticles self-assemble with high efficiency, are resistant to RNase degradation and 8M urea denaturation, and remain intact after injection into the body. Ribozyme, siRNA, miRNA, or aptamers can be fused to the nanoparticles without affecting the folding of the core or the functionality of the modules. RNA can serve as a boiling-resistant anionic polymer material to build robust polygons with tunable angles, sizes, stoichiometry and functionality. The angle of the pRNA 3WJ can be tuned from 60o to 90o or 108o, transitioning from triangle to square or pentagon. The size of RNA nanoparticles can be easily increased or decreased as shown by the construction of 5, 10, and 20 nm square nanoparticles. The vertices of the angular nanoparticles were used to incorporate siRNA, ribozyme, and fluorogenic RNA motifs. Triangular nanoparticles were used to construct supramolecular hexamer constructs as well as honeycomb shaped arrays. This technique for simple design to finely tune RNA architectures adds a new angle to RNA nanotechnology.
References:
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Keywords: Bionanotechnology, RNA Nanoparticles, Tunable Size and Properties