Poster abstracts

Poster number 19 submitted by Hunter Gill

RAZOR: a Unified Database for Respiratory Virus PCR Primers

Hunter Mathias Gill (BioHealth Informatics, Indiana University School of Informatics & Computing at IUPUI)

Abstract:
The current COVID-19 pandemic highlights the significance of human respiratory RNA viruses. PCR-based technologies have been indispensable tools in the global response to COVID-19 by providing platforms for both biological research and molecular diagnostic testing [1]. The RAZOR database was developed to help provide high-quality primers for the detection of the causative COVID-19 agent, SARS-CoV-2, as well as similar RNA-genome respiratory viruses. All RAZOR primers sets are designed with the Primer3 algorithm and cover amplicon size ranges for both conventional and RT PCR protocols. Primer sets are also binned into distinct conservation categories according to BLAST hits against a database of all known viruses. The primer sets can be accessed and downloaded from highly interactive results pages powered by igv.js [2].

RAZOR Database: https://sysbio.informatics.iupui.edu/primer_project/website/

References:
[1] Bustin SA, T Nolan. (2020). RT-qPCR testing of SARS-CoV-2: A primer. Int. J. Mol. Sci.2020 Apr; 21(8); 3004. doi: 10.3390/ijms21083004
[2] https://github.com/igvteam/igv.js/

Keywords: database, respiratory virus, PCR