Poster abstracts
Poster number 49 submitted by Lewis Rolband
Using RNA nanoring scaffolds to modulate the fluorescent properties of silver nanoclusters
Lewis Rolband (Department of Chemistry, University of North Carolina at Charlotte), Liam Yourston (Department of Physics, University of Nebraska Omaha), Caroline West (Department of Chemistry, University of North Carolina at Charlotte), Alexander Lushnikov (Nanoimaging Core Facility at the University of Nebraska Medical Center), Kirill Afonin (Department of Chemistry, University of North Carolina at Charlotte), Alexey Krasnoslobodtsev (Department of Physics, University of Nebraska Omaha)
Abstract:
Nucleic acid nanoparticles (NANPs) are self-assembling which reliably fold into sequence determined architectures. By using NANPs as a scaffold, functional moieties can be spatially oriented with defined structural relationships to one another. Upon combining DNA oligonucleotides, which templated the formation of silver nanoclusters (AgNCs), with an RNA based nanoring, the resultant inorganically modified biopolymer exhibited unexpected tunable fluorescent properties. The AgNC formation around the nanoring was found to be localized to the templating DNA strand, which resulted in the formation of 6 individual AgNCs around the hexameric RNA nanoring scaffold. The optical properties of the fluorescent AgNC-NANP complexes were characterized thoroughly using 2D analysis of excitation-emission matrices including their evolution with time of storage. Two distinct fluorescent species were observed with the emitted color being either ‘red’ (λExc/λEm = 565/623 nm) or ‘green’ (λExc/λEm = 440/523 nm). The relative stability of each of these species was reliant upon the orientation of the AgNC in respect to the NANP scaffold. ‘Red’ fluorescent species began to emit ‘green’ over a storage period, due to oxidation of the AgNC. The rate of decay from ‘red’ to ‘green’ was dramatically slower for AgNCs which were oriented towards the inside of the ring as opposed to those oriented towards the outside. The ‘red’ fluorescence of the oxidized AgNCs was restored by re-reduction of the Ag+ to Ag0. The findings presented herein display the complexity of AgNC-NANP fluorescence properties and add to the fluorescent hybrid biomaterials toolkit.
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Keywords: Silver Nanoclusters, Fluorescence, Nucleic Acid Nanoparticle