Talk abstracts
Talk on Saturday 10:30-11:00am submitted by Rhiju Das
Accelerated 3D RNA structure determination and the SARS-CoV-2 genome
Zhang, K., Zheludev, I. N. (Biochemistry Department, Stanford University), Hagey, R. J., Wu, M. T.-P., Haslecker, R. (Biochemistry Department, Stanford University), Hou, Y. J., Kretsch, R., Pintilie, G. D., Rangan, R. (Biochemistry Department, Stanford University), Kladwang, W., Li, S., Pham, E. A., Bernardin-Souibgui, C. (Biochemistry Department, Stanford University), Baric, R. S., Sheahan, T. P., D Souza, V., Glenn, J. S., Chiu, W. (Biochemistry Department, Stanford University), Das, R. (Biochemistry Department, Stanford University)
Abstract:
The discovery and design of biologically important RNA molecules has typically outpaced three-dimensional structural characterization. This talk will describe Ribosolve, a hybrid method integrating cryo-EM. biochemistry, and computer modeling, and its application to conserved segments of the SARS-CoV-2 RNA genome. The virus's frameshifting element is the smallest macromolecule (88 nt; 28 kDa) resolved by single-particle cryo-EM at subnanometer resolution to date and illustrates how rapid structure determination can lead to functional insights with implications for biomedical targeting of RNA.
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