Poster abstracts
Poster number 106 submitted by Andrew Reynolds
Rational Design of Switchable Aptamer Sensors through Structural and Thermodynamic Tuning
Andrew Reynolds (Chemistry Dept., BGSU, Center for Photochemical Sciences), Hari Timilsina (Chemistry Dept., BGSU, Center for Photochemical Sciences), Pavel Anzenbacher (Chemistry Dept., BGSU, Center for Photochemical Sciences), Xiaohong Tan (Chemistry Dept., BGSU, Center for Photochemical Sciences)
Abstract:
Fluorogenic fusion aptamer sensor design is complicated by troubleshooting with increased labor and cost. Secondary structure prediction software can help reduce the number of designs to screen experimentally. We propose using predicted equilibrium probability as an intentional design feature to create a Broccoli aptamer-based ADP sensor. This work demonstrates a more streamlined approach for fluorogenic fusion aptamer sensors by establishing landmarks to guide exploration of the sensor-transducer space.
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Keywords: Fluorogenic RNA, Aptamer, Sensor