Poster abstracts
Poster number 75 submitted by Ivy Mordick
Cell-cycle-dependent mRNA Localization at Centrosomes
Ivy X. Mordick (Department of Physiology and Cell Biology, The Ohio State University), Monish Prabhala (Department of Physiology and Cell Biology, The Ohio State University), Junnan Fang (Department of Physiology and Cell Biology, The Ohio State University)
Abstract:
Subcellular RNA localization is crucial for development by spatially regulating gene expression and function, thus establishing cell polarity, cell fate, and tissue development. Centrosomes are membranelles organelles that serve as microtubule-organizing centers (MTOCs), supporting cell division, intracellular trafficking, and cilia formation. Although the centrosomal mRNA localization to the centrosome has been widely reported, the underlying regulatory mechanisms remain elusive. Recently, using single molecular fluorescent in situ hybridization, we found that two mRNAs, Bsg25D and girdin, are localized to centrosomes in Drosophila melanogaster embryos throughout the cell cycle. Using these two centrosomal mRNAs as models, we investigated the mechanisms regulating their localization, by defining the roles of microtubule-based transport machinery, centrosome regulators, and centrosome components in mRNA trafficking to centrosomes. We found that Drosophila embryos with a cnnB4 mutation, which hinders PCM maturation, prevents Bsg25D mRNA localization to the centrosome in all stages of the cell cycle. However, when Drosophila fragile X mental retardation protein (dFMRP), an RNA binding protein implicated in regulating centrosome function, was knocked down, it resulted in an increase in Bsg25D mRNA localization, specifically during interphase. In contrast, dFMRP knockdown resulted in increased girdin mRNA localization during mitosis. These results indicate that centrosomal mRNA localization and its regulation are cell cycle-dependent, implicating their role in supporting centrosome maturation
Keywords: RNA localization, centrosome, Drosophila