Poster abstracts
Poster number 106 submitted by Kritisha Bhandari
RNA Aptamer Development for Biosensor by SPR-SELEX
Kritisha Bhandari (Department of Chemistry, Southern Illinois University Edwardsville), Sun Jeong Im (Department of Chemistry, Southern Illinois University Edwardsville), Mina Sumita (Department of Chemistry, Southern Illinois University Edwardsville)
Abstract:
E. coli outbreak is not only a problem in the United States but is a global concern. A biosensor that can detect harmful microorganisms can be a very effective approach to prevent this issue. Aptamers, single-stranded nucleotides, have high affinity towards a specific target molecule. Biosensors, when developed with an aptamer, are capable of detecting microorganisms like E. coli. An aptamer can be selected from the process of SELEX (Systematic Evolution of Ligand by Exponential Enrichment). A SELEX with surface plasmon resonance (SPR-SELEX) was recently developed in our lab to accelerate the SELEX process. In this project, the SPR-SELEX will expand to RNA aptamer selection. RNA can form more diverse structures than DNA. This research emphasizes the identification of an RNA aptamer sequence for E. coli K-12 strain.
Keywords: SELEX, SPR