Poster abstracts
Poster number 92 submitted by Mamta Kaura
Process Development for the Synthesis of Chemically Modified DNA Oligonucleotide
Mamta Kaura (Process Development Department, Nitto Denko Avecia), Arnie De Leon (Process Development Department, Nitto Denko Avecia), Parades Eduardo (Process Development Department, Nitto Denko Avecia)
Abstract:
Chemically modified oligonucleotides play a vital role in the field of medicine and biotechnology. Avecia has extensive experience in optimizing the manufacturing process of various modified oligonucleotides. Here, we present the synthesis optimization of a DNA oligonucleotide modified with hydrophobic lipid group at the 5'-end. Finding a suitable solvent system to ensure the solubility of the lipid phosphoramidite solution and the coupling mixture significantly increased the coupling efficiency thus increasing the final yield.
Keywords: Oligonucleotides, Lipid, Coupling efficiency