Talk abstracts
Talk on Saturday 11:00-11:15am submitted by Qinyu Sun
MIR100 host gene-encoded lncRNAs regulate cell cycle by modulating the interaction between HuR and its target mRNAs
Qinyu Sun, Vidisha Tripathi, Deepak Singh, Qinyu Hao, Supriya G. Prasanth (Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign), Je-Hyun Yoon, Kyung-Won Min, Sylvia Davila, Richard W. Zealy, (Department of Biochemistry and Molecular Biology, Medical University of South Carolina), Xiao Ling Li, Ashish Lal (Regulatory RNAs and Cancer Section, Genetics Branch, Center for Cancer Research, National Cancer Institute), Elin Lehrmann, Yongqing Zhang, Kevin G. Becker, Myriam Gorospe (Laboratory of Genetics and Genomics, National Institute of Aging-Intramural Research program, NIH), Susan M. Freier (Ionis Pharmaceuticals Inc, Carlsbad, CA), Kannanganattu Prasanth (Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign)
Abstract:
Long non-coding RNAs (lncRNAs) regulate vital biological processes, including cell proliferation, differentiation and development. A subclass of lncRNAs is synthesized from microRNA (miRNA) host genes (MIRHGs) due to pre-miRNA processing, and are categorized as miRNA-host gene lncRNAs (lnc-miRHGs). Presently, the cellular function of most lnc-miRHGs is not well understood. We demonstrate a miRNA-independent role for a nuclear-enriched lnc-miRHG in cell cycle progression. MIR100HG produces spliced and stable lncRNAs that display elevated levels during the G1 phase of the cell cycle. Depletion of MIR100HG-encoded lncRNAs in human cells results in aberrant cell cycle progression without altering the levels of miRNA encoded within MIR100HG. Notably, MIR100HG interacts with HuR/ELAVL1 as well as with several HuR-target mRNAs. Further, MIR100HG-depleted cells show reduced interaction between HuR and three of its target mRNAs, indicating that MIR100HG facilitates interaction between HuR and target mRNAs. Our studies have unearthed novel roles played by a MIRHG-encoded lncRNA in regulating RNA binding protein activity, thereby underscoring the importance of determining the function of several hundreds of lnc-miRHGs that are present in human genome.
Keywords: long non-coding RNA, microRNA host gene , RNA-binding proteins