Poster abstracts
Poster number 145 submitted by Trinh T Tat
High-resolution integrative analysis to define putative cytoplasmic RNA recapping sites in mammalian cells
Trinh T Tat (Department of Cardiovascular Sciences - Houston Methodist Research Institute), Dongyu Zhao (Department of Cardiovascular Sciences - Houston Methodist Research Institute), Kaifu Chen (Department of Cardiovascular Sciences - Houston Methodist Research Institute), Daniel L Kiss (Department of Cardiovascular Sciences - Houston Methodist Research Institute)
Abstract:
Multiple lines of evidence showed cytoplasmically-recapped transcripts undergo N7 methylation to generate the same m7G caps found on nuclear-capped RNAs. This mechanism remains poorly understood although several studies show that these recapped targets are involved in certain key biological processes, such as cell cycle, cellular stress response, and development. Many questions regarding cytoplasmic capping remain unanswered including how the cell identifies and selects cytoplasmic capping sites. By integrating public CAGE and ChIP-Seq datasets that mark transcription start sites (TSSs) (RNA polymerase II and 8 chromatin marks) and gene bodies (2 chromatin marks) we have developed a high-resolution integrative analysis to precisely define the locations of non-TSS-linked caps. These m7G caps are candidate cytoplasmic RNA capping sites (non-traditional 5’ m7G caps along gene bodies) in mammalian cells. 5’ RACE will be using to experimentally validate some of these putative cytoplasmic capping sites predicted by this bioinformatic approach. In addition, nanopore long read direct RNA sequencing is expected to elucidate the targets of cytoplasmic recapping and may greatly aid in defining the site selection rules for cytoplasmic capping.
Keywords: cytoplasmic recapping, CAGE, TSS