Poster abstracts
Poster number 23 submitted by Menglin Chen
Measures of single- versus multiple-round translation argue against a mechanism to ensure coupling of transcription and translation
Menglin Chen (Department of Microbiology, Ohio State Biochemistry Program, Center for RNA Biology, The Ohio State University, Columbus, OH 43210), Kurt Fredrick (Department of Microbiology, Ohio State Biochemistry Program, Center for RNA Biology, The Ohio State University, Columbus, OH 43210)
Abstract:
In prokaryotes, the synthesis of RNA and protein occurs simultaneously in the cytoplasm. A number of studies indicate that translation can strongly impact transcription, a phenomenon often attributed to physical coupling between RNA polymerase (RNAP) and the lead ribosome on the nascent mRNA. Whether there generally exists a mechanism to ensure or promote RNAP–ribosome coupling remains unclear. Here, we used an efficient hammerhead ribozyme and developed a reporter system to measure single- versus multiple-round translation in Escherichia coli. Six pairs of cotranscribed and differentially translated genes were analyzed. For five of them, the stoichiometry of the two protein products came no closer to unity (1:1) when the rounds of translation were severely reduced in wild-type cells. Introduction of mutation rpoB(I572N), which slows RNAP elongation, could promote coupling, as indicated by stoichiometric SspA and SspB products in the single-round assay. These data are consistent with models of stochastic coupling in which the probability of coupling depends on the relative rates of transcription and translation and suggest that RNAP often transcribes without a linked ribosome.
Keywords: ribosome, RNA polymerase, NusG