Poster abstracts

Poster number 5 submitted by Rolando Alvarado

Cytoplasmic recapping, a new epitranscriptomic modification targeting long non-coding RNA

Rolando Alvarado (Cardiovascular Regeneration, Houston Methodist), Daniel Kiss (Cardiovascular Regeneration, Houston Methodist)

Abstract:
Cytoplasmic recapping is an epitranscriptomic modification that adds a new 7-methylguanosine (m⁷G) cap onto RNA. Noncoding RNA share key characteristics with mRNAs and can possibly be targets for the cytoplasmic recapping machinery. Recapping of truncated ncRNA may significantly alter their stability and/or function(s). This proof of concept study utilized Clariom D Microarrays, qPCR and other molecular methods to assay RNAs harvested from control cells and those expressing a Dominant Negative Cytoplasmic Capping Enzyme (DN-cCE) which inhibits the cytoplasmic recapping machinery. Preliminary microarray data show over 1,000 ncRNAs had detectable changes when cytoplasmic capping was blocked by expressing DN-cCE. A more extensive study is required to build upon this proof of concept and to identify cytoplasmically recapped lncRNAs with greater certainty.

References:
Berger, M.R., Alvarado, R. and Daniel L. Kiss (2019) mRNA 5’ ends targeted by cytoplasmic recapping cluster at CAGE tags and select transcripts are alternatively spliced. In press: FEBS Letters.
Ulitsky, I., & Bartel, D. P. (2013). lincRNAs: genomics, evolution, and mechanisms. Cell, 154(1), 26-46.
Naemura, M., Kuroki, M., Tsunoda, T., Arikawa, N., Sawata, Y., Shirasawa, S., & Kotake, Y. (2018). The long noncoding RNA OIP5-AS1 is involved in the regulation of cell proliferation. Anticancer research, 38(1), 77-81.
Dagvadorj, A., Tan, S. H., Liao, Z., Xie, J., Nurmi, M., Alanen, K., ... & Nevalainen, M. T. (2010). N-terminal truncation of Stat5a/b circumvents PIAS3-mediated transcriptional inhibition of Stat5 in prostate cancer cells. The international journal of biochemistry & cell biology, 42(12), 2037-2046.

Keywords: Epitranscriptomic , RNA modification, cytoplasmic recapping